![]() ![]() These results allow a better planning of the embryo transfer procedure, considering low number of embryos and the choice of unilateral transfers as the ideal condition for an optimal outcome. B6129F1 presented similar results, but bilateral transfers were more effective with low number of embryos. The results achieved show that C57BL/6J transfers with a low number of embryos provide higher success rates when using unilateral transfers, but for bilateral transfers a minimum number of embryos seems to be necessary. C57BL/6J and B6129F1 embryos were freshly collected and transferred to recipient females, after overnight culture to a 2-cell stage and tested for different conditions (unilateral and bilateral surgical procedures, variable number of embryos and reciprocity between recipient mother and embryo’s genetic background). The aim of this study is to further characterize the optimal conditions to perform embryo transfer using wild type strains and particularly the bilateral transfer. This procedure consists of the transfer of mouse embryos into the oviduct of a pseudopregnant recipient female in order to obtain live pups. Embryo transfer is a common procedure in rodent facilities related to rederivation protocols, recovery of cryopreserved embryos and production of genetically engineered animals. ![]()
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